Figure 6. Gli3 mediates TLR-induced inflammation.

(A) WT and Irf3^−/− MEFs (1.5x105) were treated with transfected with 10 μg/mL Poly(I:C) for the indicated time followed by qPCR to determine GLI3 expression. (B) MM6 cells (4 × 10⁶) were transfected with shScr or shIRF3. After 24 hr, cells were stimulated with 10 μg/mL poly(I:C) for an additional 24 hr. Cells were harvested and used for RNA isolation and qPCR to determine GLI3 expression. (C) IFA-elicited macrophages from M-Gli3^−/− or WT mice (n = 14/group) were cultured in the presence or absence of 100 ng/ml LPS for 24 hours. Supernatants were used to quantify cytokine levels by ELISA. (D) WT and Gli3^−/− MEFs (1 × 10⁶ cells/ml) were plated in 0.5 ml volume in 12-well plates. Cells were treated with 1 μg/mL MPLA for 24 hr followed by ELISA to quantify cytokine secretion. MEF experiments were repeated twice with similar results.