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. 2022 Jul 21;11:e78517. doi: 10.7554/eLife.78517

Key resources table.

Reagent type (species) or resource Designation Source or reference Identifiers Additional information
Genetic reagent (Mus musculus) C57BL/6 J Taconic B6-F/M
Genetic reagent (Mus musculus) Cldn5(BAC)-GFP Laviña et al., 2018 N/A
Genetic reagent (Mus musculus) Cldn5 iECKO This paper N/A See Figure 3—figure supplement 1 and Methods-Animals
Genetic reagent (Mus musculus) Cldn5fl/fl; Rosa26lox-STOP-lox-YFP; Cdh5CreERT2 This paper N/A See Methods-Animals
Antibody Mouse monoclonal anti-GAPDH Millipore MAB374 1:1,000
Antibody Rat monoclonal anti-CD31 BD Biosciences 553370 1:100
Antibody Goat polyclonal anti-CD31 R&D Systems AF3628 1:100
Antibody Goat polyclonal anti-VE-Cadherin R&D Systems AF1002 1:100, 1:1000
Antibody Chicken polyclonal anti-GFP Abcam Ab13970 1:100
Antibody Rabbit polyclonal anti-Claudin5 ThermoFischer Scientific 341600 1:100, 1:1000
Antibody Rabbit polyclonal anti-ZO-1 ThermoFischer Scientific 617300 1:100, 1:1000
Antibody Rabbit polyclonal anti-Occludin ThermoFischer Scientific 711500 1:1,000
Antibody Rabbit monoclonal anti-JAM-A Martìn-Padura et al., 1998 N/A 1:1000
Antibody Rabbit polyclonal anti-Cingulin Cardellini et al., 1996 N/A 1:1000
Antibody Goat polyclonal anti-ESAM R&D Systems AF2827 1:1000
Antibody Goat polyclonal anti-collagen IV Merck Millipore AB789 1:100
Antibody Mouse monoclonal anti-αSMA FITC Sigma Aldrich F3777 1:100
Antibody Mouse monoclonal anti- αSMA Cy3 Sigma Aldrich C6198 1:100
Antibody Rat monoclonal Anti-CD16/32 ThermoFischer Scientific 14-0161-85 1:100
Antibody Rat monoclonal Anti-CD31 FITC BD Biosciences 553372 1:50
Antibody Rat monoclonal Anti-CD45 APC BioLegend 103112 1:50
Antibody Rat monoclonal Anti-Lyve1 eFluor 660 ThermoFischer Scientific 50–0443082 1:50
Antibody Donkey polyclonal anti-rat alexa 488 ThermoFischer Scientific A21208 1:400
Antibody Donkey polyclonal anti-rat alexa 594 ThermoFischer Scientific A21209 1:400
Antibody Donkey polyclonal anti-rabbit alexa 488 ThermoFischer Scientific A21206 1:400
Antibody Donkey polyclonal anti-rabbit alexa 568 ThermoFischer Scientific A10042 1:400
Antibody Donkey polyclonal anti-goat alexa 647 ImmunoResearch Laboratories 705-605-147 1:400
Antibody Donkey polyclonal anti-chicken alexa 488 ImmunoResearch Laboratories 703-545-155 1:400
Antibody Sheep polyclonal anti-mouse HRP Cytiva NA931 1:10,000
Antibody Sheep polyclonal anti-rabbit HRP Cytiva NA934 1:10,000
Sequence-based reagent Cldn5 probe ACD Bio 491611-C2
Sequence-based reagent 3-plex negative control probes ACD Bio 320871
Sequence-based reagent 3-plex positive control probes ACD Bio 320811
Sequence-based reagent GAPDH ThermoFischer Scientific Mm99999915_g1
Sequence-based reagent Cldn5 ThermoFischer Scientific Mm00727012_s1
Sequence-based reagent Cdh5 ThermoFischer Scientific Mm00486938_m1
Sequence-based reagent Tjp1 ThermoFischer Scientific Mm01320638_m1
Sequence-based reagent Ocln ThermoFischer Scientific Mm00500912_m1
Sequence-based reagent F11r ThermoFischer Scientific Mm00554113_m1
Sequence-based reagent Cgn ThermoFischer Scientific Mm01263534_m1
Sequence-based reagent Esam ThermoFischer Scientific Mm00518378_m1
Peptide, recombinant protein Collagenase IV Worthington LS004183
Peptide, recombinant protein DNase I Worthington LS006333
Peptide, recombinant protein HRP SigmaAldrich 77332
Commercial assay or kit RNAscope Fluorescent Multiplex Assay ACD Bio 322340, 320851
Commercial assay or kit RNeasy Plus kit Qiagen 74034
Commercial assay or kit iScript Adv cDNA Kit for RT-qPCR Bio-Rad 1725038
Chemical compound, drug Tamoxifen SigmaAldrich T5648
Chemical compound, drug 4-hydroxytamoxifen SigmaAldrich H7904
Chemical compound, drug Oxazolone SigmaAldrich E0753
Chemical compound, drug Histamine SigmaAldrich H7125
Other Live/Dead near IR cell stain ThermoFischer Scientific L10119 See Materials and methods-Ear dermal single cell isolation
Other Phosphatase inhibitor cocktail Roche 04906837001 See Materials and methods-Western blot analysis
Other LDS sample buffer Invitrogen NP0007 See Materials and methods-Western blot analysis
Other Sample reducing agent Invitrogen NP0009 See Materials and methods-Western blot analysis
Other MOPS SDS running buffer Invitrogen NP0001 See Materials and methods-Western blot analysis
Other PVDF membrane Thermofischer Scientific 88518 See Materials and methods-Western blot analysis
Other NuPAGE transfer buffer Novex NP006 See Materials and methods-Western blot analysis
Other RNAlater ThermoFischer Scientific AM7024 See Materials and methods-quantitative PCR
Other 2000 kDa FITC Dextran SigmaAldrich FD2000S See Materials and methods-permeability analysis
Other 2000 kDa TRITC Dextran Lysine Fixable ThermoFischer Scientific D7139 See Materials and methods-permeability analysis
Other 10 kDa TRITC Dextran ThermoFischer Scientific D1817 See Materials and methods-permeability analysis
Other 4 kDa TRITC Dextran Tdb labs TD4 See Materials and methods-permeability analysis
Other 10 kDa FITC Dextran Tdb labs FD10 See Materials and methods-permeability analysis
Other 70 kDa TRITC Dextran Tdb labs TD70 See Materials and methods-permeability analysis
Other 2000 kDa FITC Dextran lysine fixable Tdb labs FLD2000 See Materials and methods-permeability analysis
Other Anti-Isolectin GS-IB4 Molecular Probes I32450 See Materials and methods-Immunohistochemistry