Appendix 1—video 2. High-magnification movie of iBAT fixed-frozen tissue section from cold-exposed mice stained for GPNMB (green), MHCII (purple), and PLIN1 (red).

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Nuclei were counterstained with DAPI. Video shows sequential addition of the following elements: (1) image with immune marker channels turned off to show the outline of the effercytosis site (ES). (2) GPNMB+ cells (green) in the ES. (3) MHCII+ cells (purple) in the ES. Note that both GPNMB+ cells and MHCII+ cells surround cellular vacancies.