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. 2022 Jul 20;12:868415. doi: 10.3389/fonc.2022.868415

Figure 9.

Figure 9

Biological function of CD81 in glioblastoma cells. (A) The relative CD81 mRNA levels in glioblastoma and HA1800 cells were measured by qRT-PCR. (B) Western blot analysis confirmed that CD81 siRNA inhibited CD81 expression. NC-treated cells served as controls. (C) The relative CD81 mRNA expression levels measured by qRT-PCR in NC-treated and Si1-CD81-treated glioblastoma cells. (D) The CCK-8 assay was used to detect the effect of CD81 knockdown on the proliferation of U251 and U118 cells. (E) Western blot analysis of SOX10 and Nanog in NC-treated and Si1-CD81-treated U118 cells suggested a role of CD81 in stemness regulation of glioblastoma cells. (F, G) Representative images and quantitative analysis of the wound healing assay recorded at 0, 12, and 24 h. (H, I) Representative images of the transwell assay. Cell staining results and migration cell quantification are shown. (J, K) Apoptosis detection using an Annexin V-FITC/PI apoptosis detection kit. ****p < 0.0001, ***p < 0.001, **p < 0.01 *p < 0.05.