TABLE 1.
Sequence analysis of spontaneous mutations in ebgR
| Growth-dependent mutations
|
Adaptive mutations
|
|||
|---|---|---|---|---|
| Mutationa | Result | Mutation | Result | |
| C130→A | Ala→Glu | A126→C | Met→Leu | |
| C130→A | Ala→Glu | Δ130–142 | ||
| A138→G | Lys→Glu | T136→G | Leu→Arg | |
| A144→T | Ile→Phe | C157→A | Ala→Asp | |
| C148→A | Ala→Glu | C157→A | Ala→Asp | |
| ΔC151 | Frameshift | C157→A | Ala→Asp | |
| G153→T | Ochre | T165→C | Ser→Pro | |
| T165→C | Ser→Pro | T177→A | Val→Glu | |
| C172→A | Ala→Glu | A183→T | Arg→Cys | |
| C172→A | Ala→Glu | A183→T | Arg→Cys | |
| T178→G | Val→Gly | G184→A | Arg→Lys | |
| T180→A | Ser→Thr | G184→A | Arg→Lys | |
| C181→A | Ser→Tyr | A199→C | Asp→Ala | |
| C181→T | Ser→Phe | T200→G | Asp→Glu | |
| G184→A | Arg→Lys | T200→G | Asp→Glu | |
| T187→G | Val→Gly | A210→G | Asn→Asp | |
| A191→C | Leu→Phe | A210→G | Asn→Asp | |
| A199→C | Asp→Ala | G213→A | Val→Met | |
| A210→G | Asn→Asp | T insertion after 220 | Frameshift | |
| G213→A | Val→Met | C226→A | Thr→Lys | |
| G219→T | Ochre | G243→T | Amber | |
| C250→A | Ala→Asp | G243→T | Amber | |
| ΔG260 | Frameshift | G243→T | Amber | |
| C266→A | Ochre | G252→T | Ochre | |
| C266→G | Amber | C266→A | Ochre | |
| C266→G | Amber | A285→T | Ochre | |
| C309→T | Ochre | C325→A | Ala→Asp | |
| C309→T | Ochre | C332→A | Ochre | |
| C338→G | Amber | G382→T | Arg→Leu | |
| G351→T | Amber | G403→T | Cys→Phe | |
| Δ368–381 | G403→T | Cys→Phe | ||
| C368→A | Ochre | C404→A | Opal | |
| ΔG393 and C397→A | Frameshift | A462→T | Ochre | |
| C404→A | Opal | ΔC520 | Frameshift | |
| G405→T | Ochre | ΔC520 | Frameshift | |
| ΔG413 | Frameshift | ΔC520 | Frameshift | |
| T451→A | Ochre | T543→C | Cys→Arg | |
| T451→A | Ochre | G582→A | Ala→Thr | |
| T451→G | Opal | G615→T | Ochre | |
| Δ454–463 | Δ622–632 | |||
| A462→T | Ochre | C639→T | Amber | |
| Δ485–495 | C639→T | Amber | ||
| C578→A | Ochre | C639→T | Amber | |
| ΔC583 | Frameshift | C639→T | Amber | |
| G615→T | Ochre | C639→T | Amber | |
| C639→T | Amber | 2-bp duplication after 647 | Frameshift | |
| C639→T | Amber | G678→T | Amber | |
| C639→T | Amber | T695→G | Asp→Glu | |
| T insertion after 686 | Frameshift | 5-bp duplication after 715 | Frameshift | |
| G702→T | Amber | ΔT722 | Frameshift | |
| G702→T | Amber | C735→T | Ochre | |
| G717→T | Ochre | T742→G | Val→Gly | |
| Duplication 760–811 | G750→T | Amber | ||
| G761→A | Opal | Δ760–809 | ||
| Δ767–818 | G761→A | Opal | ||
| G792→A | Ochre | G766→C | Gly→Ala | |
| ΔA872 | Frameshift | T insertion after 769 | Frameshift | |
| Δ904–918 | G769→T | Gly→Val | ||
| ΔT917 | Frameshift | C775→T | Ser→Phe | |
| C919→A | Amber | C784→A | Amber | |
| C919→A | Amber | G819→T | Ochre | |
| Δ936–1019 | T insertion after 824 | Frameshift | ||
| C951→T | Opal | T838→C | Leu→Pro | |
| G993→T | Amber | G852→T | Asp→Tyr | |
| G993→T | Amber | T865→C | Ile→Ser | |
| G1002→A | Gly→Arg | G868→A | Gly→Asp | |
| G1003→A | Gly→Glu | G868→T | Gly→Val | |
| G1012→A | Gly→Ser | C929→A | Ser→Arg | |
| ΔA1017 | Frameshift | Δ931–945 | ||
| 4-bp duplication after 1018 | A937→G | Asp→Gly | ||
| Δ1019–1022 | T956→A | Phe→Leu | ||
| Δ1047–1048 | G993→T | Amber | ||
| Δ1083–1092 | G993→T | Amber | ||
| G993→T | Amber | |||
| G993→T | Amber | |||
| G1003→A | Gly→Glu | |||
| A insertion after 1029 | Frameshift | |||
| ΔGG 1044–1045 | ||||
| A1080→T | Ochre | |||
| T1084→A | Ochre | |||
| ΔG1095 | Frameshift | |||
| ΔG1095 | Frameshift | |||
| C1099→G | Thr→Arg | |||
| AA1211,1212→GC | Ochre | |||
a
Numbers refer to positions in the ebg operon as numbered in GenBank accession no. M64441.