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. 2022 Aug 3;12:13361. doi: 10.1038/s41598-022-17579-0

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Establishment of miR-101a over-expressing mouse model in vivo. Three-week-old R26/101a male and female mice were maintained on regular (reg) or fed a doxycycline-supplemented (dox) diet for five weeks. Indicated tissues were assessed by RT-qPCR analysis. Administration of doxycycline is anticipated to induce miR-101a and GFP expression. An expression screen for GFP (A) and miR-101-5p (B) in eight tissues derived from eight-week-old female mice (n = 2). Quantitative expression of Col1a1, GFP, Akt1, Ezh2, Sp7/Osx, and Adipoq (C) and miR-101-5p and miR-218 (D) in bone (femora), skeletal muscle (thigh area), and fat (visceral) of eight-week-old male mice (n = 4 to 5, mean ± STD). **p < 0.01 and ***p < 0.001. We note that miR-218 was arbitrarily included as a representative miRNA to indicate selectivity in the differences in expression of miR-101-5p across tissues. MiRNA expression levels are presented using miR-103a-3p as internal control for normalization.