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. 2022 Aug 3;12:13361. doi: 10.1038/s41598-022-17579-0

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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miR-101a expression is induced in male mice. Femora (left) were collected from R26 control, miR-101a control, and miR-101a over-expressing (R26/101) male mice treated with doxycycline from inception to sacrifice (8 weeks). RNA (A) and miRNA (B) RT-qPCR analysis of all three mouse cohorts as well as RT-qPCR assessment for GFP in two control groups (C) (same values as in A) (n = 8 to 10, mean ± STD) are shown. **p < 0.01 and ****p < 0.0001. We note that miR-191-5p was arbitrarily included as a representative miRNA to reflect selectivity in modulations in the expression of miR-101-5p. MiRNA expression levels are presented using miR-103a-3p as internal control for normalization.