TABLE 3.
Effect of GlnK on the regulation of K. pneumoniae pnifH
| Strain | Relevant genotype
|
β-Galactosidase activitya
|
||
|---|---|---|---|---|
| Chromosome | Plasmid | −Nb | +Nc | |
| UNF923(pCC46) | Wild type | nifL+ nifA+ | 618 | 34 |
| UNF3440(pCC46) | glnB | nifL+ nifA+ | 498 | 31 |
| UNF3433(pCC46) | glnK | nifL+ nifA+ | 161 | 33 |
| UNF3433(pCC46, pRJ44) | glnK | nifL+ nifA+, glnK+ | 613 | 109 |
| UNF923(pCC46, pRJ44) | Wild type | nifL+ nifA+, glnK+ | 609 | 56 |
| UNF923(pCC47) | Wild type | nifA+ | 1,336 | 1,124 |
| UNF3440(pCC47) | glnB | nifA+ | 1,075 | 967 |
| UNF3433(pCC47) | glnK | nifA+ | 1,045 | 1,233 |
a
Expressed from the chromosomal nifH::MudAplac fusion (in Miller units).
b
−N, nitrogen-limiting medium.
c
+N, nitrogen-sufficient medium.