FIG. 4.

Effects of temperature on the stability of P. furiosus ADH. The enzyme (3.6 mg/ml in 50 mM Tris-HCl [pH 7.8] containing 2.0 mM dithiothreitol) was incubated in stoppered glass vials at 85°C (●) or 95°C (□). Samples were removed at intervals and assayed by the NADP-dependent oxidation of ethanol at 80°C.