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Purification of ADH from P. furiosus
| Purification step | Total protein (mg) | Activity (U) | Sp act (U/mg) | Purification (fold) | Recovery (%) |
|---|---|---|---|---|---|
| Cell extract | 7,280 | 4,030 | 0.55 | 1 | 100 |
| DEAE-Sepharose Fast Flow | 1,836 | 2,851 | 1.55 | 2.8 | 71 |
| Hydroxyapatite | 632 | 1,491 | 2.36 | 4.3 | 37 |
| Phenyl-Sepharose | 71 | 2,103 | 29.6 | 53.9 | 52 |
| Superdex 200 | 29 | 1,973 | 68.0 | 124.0 | 49 |
| Q-Sepharose High Performance | 19.2 | 1,388 | 72.3 | 131.0 | 34 |
