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Metal content of P. furiosus ADH
| Treatment | Avg metal content ± SD (g-atoms/subunit)a
|
Avg sp act (U/mg) ± SD | Activity (%) | |
|---|---|---|---|---|
| Fe | Zn | |||
| None | 1.1 ± 0.2 | 1.4 ± 0.3 | 29 ± 2 | 100 |
| Dialysis | ||||
| −EDTA | 0.9 ± 0.1 | 0.8 ± 0.2 | 24 ± 2 | 83 |
| +EDTA | 0.4 ± 0.1 | 0.8 ± 0.2 | 14.5 ± 2 | 50 |
Of the enzyme as purified and after dialysis in the presence and absence of EDTA as described in Materials and Methods.
