TABLE 5.
Substrate specificity of P. furiosus ADH
| Substrate | Activity (U/mg) | % |
|---|---|---|
| Alcohols (60 mM) | ||
| Methanol | 0 | 0 |
| Ethanol | 18.2 | 100a |
| 1-Propanol | 28.5 | 156 |
| 2-Propanol | 0 | 0 |
| 1-butanol | 30.8 | 169 |
| Isobutanol | 22.2 | 122 |
| 1-Pentanol | 31.4 | 172 |
| 1-Hexanol | 17.4 | 95 |
| 1-Heptanol | 15.7 | 86 |
| 1-Octanol | 10.5 | 57 |
| 1,3-Propanediol | 1.1 | 6 |
| 2-Phenylethanol | 13.8 | 76 |
| Tryptophol (10 mM) | 2.1 | 11 |
| Glycerol | 0 | 0 |
| d-Sorbitol | 0 | 0 |
| Aldehydes (2 mM) | ||
| Acetaldehyde | 8.4 | 46 |
| Phenylacetaldehyde | 13.8 | 76 |
| Methylglyoxal | 4.9 | 27 |
Equals 18.2 U/mg at 80°C.