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. 1999 Feb;181(4):1163–1170. doi: 10.1128/jb.181.4.1163-1170.1999

TABLE 5.

Substrate specificity of P. furiosus ADH

Substrate Activity (U/mg) %
Alcohols (60 mM)
 Methanol 0 0
 Ethanol 18.2 100a
 1-Propanol 28.5 156
 2-Propanol 0 0
 1-butanol 30.8 169
 Isobutanol 22.2 122
 1-Pentanol 31.4 172
 1-Hexanol 17.4 95
 1-Heptanol 15.7 86
 1-Octanol 10.5 57
 1,3-Propanediol 1.1 6
 2-Phenylethanol 13.8 76
 Tryptophol (10 mM) 2.1 11
 Glycerol 0 0
d-Sorbitol 0 0
Aldehydes (2 mM)
 Acetaldehyde 8.4 46
 Phenylacetaldehyde 13.8 76
 Methylglyoxal 4.9 27
a

Equals 18.2 U/mg at 80°C.