TABLE 6.
Kinetic parameters of ADH from P. furiosus
| Substrate (mMa) | Cosubstrate (mM) | Avg apparent Km (mM) ± SDb | Apparent Kcat (min−1) | Apparent Kcat/apparent Km (min−1 mM−1) |
|---|---|---|---|---|
| Ethanol (13–130) | NADP (0.4) | 29.4 ± 2.5 | 1,150 | 39 |
| Acetaldehyde (0.05–2) | NADPH (0.25) | 0.17 ± 0.02 | 329 | 1,940 |
| NADP (0.03–0.4) | Ethanol (91) | 0.071 ± 0.003 | 945 | 13,300 |
| NAD (0.1–0.6) | Ethanol (91) | 20.0 ± 2.0 | 144 | 7 |
| NADPH (0.03–0.25) | Acetaldehyde (2.0) | 0.071 ± 0.003 | 378 | 5,320 |
| 2-Phenylethanol (2–40) | NADP (0.4) | 8.0 ± 0.2 | 540 | 69 |
| Tryptophol (1–10) | NADP (0.4) | 6.3 ± 0.1 | 99 | 16 |
| Phenylacetaldehyde (0.05–2) | NADPH (0.3) | 0.091 ± 0.003 | 621 | 6,820 |
| Methylglyoxal (0.07–2) | NADPH (0.3) | 0.2 ± 0.02 | 221 | 1,100 |
a
Concentration range used to determine the kinetic constants.
b
For the corresponding substrate, not the cosubstrate.