TABLE 7.
Comparison of P. furiosus ADH with other archaeal ADHs
| Property | P. furiosus | ES-1 | T. litoralis | T. zilligii | S. solfataricus |
|---|---|---|---|---|---|
| Mol wt (structure) | 48,000 (α6) | 46,000 (α4) | 48,000 (α4) | 46,700 (α4) | 37,580 (α2) |
| Metal (g-atoms/mol) | Fe (0.9) | Fe (0.95) | Fe (0.45) | NDa | Zn (2) |
| Zn (0.8) | |||||
| Electron carrier | NADPH | NADPH | NADPH | NADPH | NADH |
| Apparent Km, alcohol (mM) | 29.4 | 8.0 | 11.0 | 10.0 | 0.019 |
| Apparent Km, aldehyde (mM) | 0.17 | 0.25 | 0.4 | 0.13 | 0.003 |
| Apparent Vmax (U/mg) | 72 | 62 | 32 | 3.2 | 3.9 |
| Optimal pH | 9.4–10.2 | 8.8–10.4 | 8.8 | 6.8–7.0 | 7.5–8.5 |
| Optimal temp (°C) | >95 | >95 | 85 | 85 | >95 |
| Stability (t1/2, h) | 160 (85°C), 7 (95°C) | 35 (85°C), 4 (95°C) | 5 (85°C), 0.3 (96°C) | 0.3 (80°C) | 5 (70°C), 3 (85°C) |
| Reference(s) | This work | 37 | 34 | 31 | 2, 50 |
a
ND, not determined.