Skip to main content
. 2022 Jul 19;32(39):2204692. doi: 10.1002/adfm.202204692

Figure 3.

Figure 3

Expression of the reporter gene and DS mRNA delivered by 4N4T‐LNPs. A–C) 293T cells, DC2.4 and BMDCs were transfected with 4N4T‐GFP mRNA and then harvested after 24 h. GFP+ cells were analyzed and quantified using flow cytometry. D,E) In vivo expression of FLuc mRNA delivered by 4N4T‐LNPs. Male BALB/c mice were intramuscularly injected with 20 µg of FLuc mRNA encapsulated in 4N4T‐LNPs. Bioluminescence was measured 8 h later in an IVIS imaging system. F) MFI of GFP and value of bioluminescence were all converted into multiples of the negative control. Core A: MIC1, MIC2; Core B: MIC 5, MIC6; Core C: MIC3, MIC4. G) The concentration of S protein in the cell culture was measured using ELISA after 293T cells had been incubated with 4N4T‐DS mRNA vaccines for 24 h. All data are presented as the mean ± SD (n = 3). Statistical significance was analyzed by one‐way ANOVA. (ns or unmarked, not significant; *, p  < 0.05; **, p  < 0.01; ***, p  < 0.001; ****, p  < 0.0001).