TABLE 2.
P. aeruginosa PAO1 cellular and external AI concentrations
| Compound | External concna | Cellular concnb | Ratio of cellular to external concnc |
|---|---|---|---|
| [3H]3OC12-HSL | 69.1 ± 6.03 nM | 231 ± 16.8 nM | 3.35 |
| [3H]C4-HSL | 56.4 ± 6.93 nM | 51.9 ± 11.4 nM | 0.92 |
| [14C]ethylene glycol | 2.10 ± 0.07 mM | 2.00 ± 0.06 mM | 0.96 |
a
Calculated from the amount of radioactivity remaining above the silicone fluid after centrifugation. Values are the averages ± SDs from three or four experiments.
b
Calculated from the amount of radioactivity centrifuged through silicone fluid and the cell volume, with a correction for the small amount of extracellular material carried through the silicone fluid (measured with [14C]dextran). Values are the averages ± SDs from four independent experiments.
c
An unpaired t test determined that the ratio of 4.4 for 3OC12-HSL was significantly higher than the ratio of 0.92 for C4-HSL (P < 0.001) and the ratio of 0.96 for ethylene glycol (P < 0.001).