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Turnover of HemA1–18-LacZ is not regulated by heme limitation. A fusion construct that expresses the HemA1–18-LacZ hybrid protein was introduced into an S. typhimurium hemL mutant background (TE2713). Two cultures of this strain (either adapted to heme limitation or not heme limited) were grown and analyzed as described in the legends to Fig. 3 and 6.
