TABLE 1.
Strains of E. coli and phages and plasmids used
| Strain, plasmid, or phage | Relevant genotype | Source or reference |
|---|---|---|
| E. coli | ||
| DH5α | Δ(argF-lac)U169 (φ80ΔlacZM15) recA | 26 |
| IMW157 | AN387 dcuC::mini-Tn10(Camr) | 37 |
| JRG1788 | MC1000 [λAΦZ.4(frdA′-lacZ)] | 30 |
| JRG1938 | RK4353 [λRZ5::fumA′-lacZ] | 35 |
| JRG2814 | AN387 dcuA::spc dcuB::kan | 29 |
| JRG3351 | MC4100 [λRS45::dctA′-lacZ] | 6 |
| JRG3835 | MC4100 [λRS45::dcuB′-lacZ] | 12 |
| JRG3983 | JRG3835 dcuS::mini-Tn10 | This work |
| JRG3984 | JRG3351 dcuS::mini-Tn10 | This work |
| JRG3985 | JRG1788 dcuS::mini-Tn10 | This work |
| JRG3986 | JRG1938 dcuS::mini-Tn10 | This work |
| MC4100 | Δ(argF-lac)U169 rpsL | 28 |
| TG1 | Δ(lac-pro) lacIq lacZΔM15 | 10 |
| SCA1 | MC4100 [λRS45::dcuB′-lacZ] dcuA::spc dcuB::kan | This work |
| SCA2 | MC4100 [λRS45::dcuB′-lacZ] dcuA::spc dcuB::kan dcuC::mini-Tn10(Camr) | This work |
| SCA3 | MC4100 dcuS::mini-Tn10 | This work |
| Phages | ||
| P1vir1 | 24 | |
| λNK1098 | 34 | |
| Plasmids | ||
| pGS78 | dcuSR | 13 |
| pGS1179 | pSU19 + 1.1-kb SalI dcuS′ fragment | This work |
| pGS1180 | pGS1179 + 2.5-kb SphI-HindIII ′dcuS-dcuR fragment | This work |
| pHSG576 | 33 | |
| pLH21 | J. K. Broome-Smith | |
| pPG1 | pSU18 + 4.3-kb PstI dcuS::mini-Tn10 fragment | This work |
| pPG2 | pHSG576 + 3.5-kb EcoRI-HindIII dcuS-dcuR fragment | This work |
| pSU18/19 | 22 | |
| pYZ4 | 36 |