FIG. 2.

Inhibition of Hfr × F⁻ recombination as a function of increasing MucA′B cell concentration. GY9427 (F⁻ leu::Tn10 ΔumuDC) (open symbol) and its derivative carrying plasmid pGY9411 (P_tac::mucA′B) (filled symbols) were mated with GY306 (HfrH), and selection was for Leu⁺ Str^r recombinants. Prior to mating, expression of MucA′B in the recipient bacteria was induced with IPTG as described in Table 4, footnote a, so as to attain the MucA′B cell concentrations indicated on the abscissa.