FIG. 4.

Synergistic inhibitory action of MucA′B and UmuD′C on recombination. GY9427 (F⁻ leu::Tn10 ΔumuDC) (left panel) and GY9461 (F⁻ leu::Tn10 ΔumuDC/o₁^cumuD′C) (right panel) and their derivatives carrying plasmid pGY9411 (P_tac::mucA′B) were mated with GY306 (HfrH). Prior to mating, expression of MucA′B in the recipients was induced with 0 or 500 μM IPTG. Open bars, recipients without MucA′B; gray and dark bars, recipients expressing MucA′B from a P_tac promoter at basal or IPTG-induced levels, respectively.