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RecA[UmuR] proteins are sensitive to recombination inhibition by MucA′B. Derivatives of GY9054 (F− leuB6 ΔrecA ΔumuDC/mini-F-recA+, -recA410, -recA411, or -recA415) and of GY9743 (F− leuB6 recA+ ΔumuDC/mini-F-recA1730) carrying plasmid GY9411 (Ptac::mucA′B) (gray and dark bars) or the vector pTrc99A (open bars) were crossed with GY10229 (HfrH). Prior to mating, expression of MucA′B in the recipients was induced with 0 (gray bars) or 500 (dark bars) μM IPTG.
