Table 2.

Encephalitozoon cuniculi testing

	Case 1	Case 2	Case 3
Serology (IgG)	1:64*	NA	1:4096 (2014) then 1:256 (2018)*
PCR	• Aqueocentesis fluid, positive † • Lens material, positive, strain II ‡	• Phacoemulsified lens fluid, positive § • Urine negative ¶	Paraffin scrolls of enucleated eye (OS), positive ¶
Histopathology	Lens capsule: Gram-positive, Ziehl–Neelsen acid-fast positive ∞	NA	Globe: intralenticular organisms, Gram-positive, variably acid-fast, Luna stain positive (see Figure 3) ¶

^*University of Miami Avian & Wildlife Laboratory

^†IDEXX Reference Laboratories

^‡Department for Pathobiology, Veterinary University Vienna

^§Athens Veterinary Diagnostic Laboratory, University of Georgia

^¶Comparative Pathology Laboratory, University of California, Davis

^∞Comparative Ocular Pathology Laboratory of Wisconsin

NA = not available