Table 1.
Primer sequences for quantitative reverse transcription-polymerase chain reaction
|
Genes
|
|
Sequence (5′– 3′)
|
Length (bp)
|
| MIP-1α | F | GCTTCTCCTATGGACGGCAA | 20 |
| R | TGCCGGTTTCTCTTGGTCAG | 20 | |
| MIP-1β | F | CTGCTTCTCTTACACCTCCCG | 21 |
| R | AAAGGCTGCTGGTCTCATAGT | 21 | |
| VEGF | F | GCGGATCAAACCTCACCAAAG | 21 |
| R | TTGGTCTGCATTCACATCTGCT | 22 | |
| PDGF | F | CTCTGCTGCTACCTGCGTCT | 20 |
| R | TCGGGTCATGTTCAAGTCCA | 20 | |
| β-actin | F | CTCTGTGTGGATTGGTGGCT | 20 |
| R | CGCAGCTCAGTAACAGTCCG | 20 |
MIP: Macrophage inflammatory protein; VEGF: Vascular endothelial-derived growth factor; PDGF: Platelet-derived growth factor; F: Forward primer; R: Reverse primer.