REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
Mouse anti-FLAG M2	Sigma-Aldrich	Cat# F3165; RRID:AB_259529
Goat anti-Mouse IgG(H+L), Human ads-Alexa Fluor 488	Southern Biotech	Cat# 1031-30; RRID:AB_2794315
Goat anti-Human IgG Fc-Alexa Fluor 647	Southern Biotech	Cat# 2048-31; RRID:AB_2795692
Biological samples/viruses
SARS-CoV-2 virus		strain USA-WA1-2020
Bacterial strains
Mix & Go Competent Cells - Strain Zymo 10B	Zymo Research	T3019
Chemicals, peptides, and recombinant proteins
Superior Broth	AthenaES	0105
Expi293 Expression Medium	Gibco	A1435102
DMEM, high glucose, pyruvate	Gibco	11995065
Fetal Bovine Serum	Gibco	26140079
Opti-MEM I Reduced Serum Medium, GlutaMAX Supplement	Gibco	51985091
Penicillin-Streptomycin (5,000 U/mL)	Gibco	15070063
Lipofectamine 3000 Transfection Reagent	Invitrogen	L3000015
Bovine Serum Albumin	Sigma-Aldrich	A3294
5X SYPRO Orange Protein Gel Stain	Supelco	S5692
Surfactant P20	Cytiva	BR100054
Commercial kits and enzymes
T7 DNA Ligase	NEB	M0318S
AarI (2 U/μL)	Thermo Fisher	ER1582
T4 DNA Ligase	NEB	B0202S
Promega Wizard SV 96 Plasmid DNA Purification Kit	Promega	A2250
ExpiFectamine 293 Transfection Kit	Gibco	A14525
Mycoplasma Detection Kit	Southern Biotech	13100-01
Pierce HRV 3C Protease Solution Kit	Thermo Fisher	88946
Deposited data
Spike Display data	This paper	Data S1
Experimental models: Cell lines
HEK293T	ATCC	CRL-3216; RRID:CVCL_0063
Vero E6	ATCC	CRL-1586; RRID:CVCL_0574
Expi293F Cells	Thermo Fisher	A14527; RRID:CVCL_D615
Oligonucleotides
Synthetic DNA (eBlocks) for cloning	Integrated DNA Technlologies	NA
Recombinant DNA
SARS-CoV-2 S HexaPro	Hsieh et al., 2020	Addgene #154754
Spike Display Part Spacers	Javanmardi et al., 2021	Addgene #172727-172733
Spike Display DO	Javanmardi et al., 2021	Addgene #172721-172726
Software and algorithms
FlowJo v9	BD	NA
SA3800 Spectral Cell Analyzer Software	SONY	NA
MATLAB (2021b)	MathWorks	https://matlab.mathworks.com/
ChimeraX 1.1	UCSF	https://www.cgl.ucsf.edu/chimerax/download.html
GraphPad Prism 9	GraphPad	https://www.graphpad.com/scientific-software/prism/
Octet Data Analysis software (v11.1)	FortéBio	NA
Public datasets
Antibody binding data	Javanmardi et al., 2021	Data S1 (“Binding” tab)
Other
384-well Echo Source Plate	Biolab	PP-0200
96-well Clear Round Bottom 2.2 mL Polypropylene Deep Well Plate (Sterile)	Axygen	P-2ML-SQ-C-S
Protein G magnetic beads	Promega	G7471
Protein A Agarose	Thermo Fisher	15918014
Strep-Tactin Superflow resin	IBA	2-1206-025
Superose 6 increase 10/300	GE healthcare	GE29-0915-96
Cell Culture Dishes (10 cm)	VWR	10062-880
Cell Culture Plate (6-well)	VWR	10861-696
Cell Culture Plate (12-well)	VWR	10861-698
96-well qPCR plate	Corning	AXYPCR96LC480WNF
black, μCLEAR flat-bottom 96-well plate	Greiner Bio-One	655096
LUNA-II Automated Cell Counter	Logos biosystems	L40002
SA3800 Spectral Analyzer	SONY	NA
Echo 525 Acoustic Liquid Handler	Beckman Coulter	NA
Tecan Fluent robotic liquid handler	Tecan	NA
Roche LightCycler 480 II	Roche	NA
Octet Anti-Mouse Fc Capture (AMC) Biosensors	FortéBio	18-5088
Octet RED96e	FortéBio	NA
QPix 420 Colony Picker	Molecular Devices	NA
Cytation 7	BioTek	NA
Detailed bench protocols for Spike Display assays	Javanmardi et al., 2021	Methods S1 in Javanmardi et al., 2021