Table I.
Gene primer sequences for mouse used for quantitative real-time polymerase chain reaction.
| Genes | Forward primers | Reverse primers |
|---|---|---|
| Adipoq | CCCCGGAACCCCTGGCAGGAAAG | GGGTCTCCAGCCCCACACTGAACG |
| Aggrecan | GGAGACCCAGACAGCAGAAACAAC | GCAGGTGGCTCCATTCAGACAAG |
| Alp | CCAGAAAGACACCTTGACTGTGG | TCTTGTCCGTGTCGCTCACCAT |
| Cebpα | TCGGTGGACAAGAACAGCAACG | CGGTCATTGTCACTGGTCAACTCC |
| Col-II | GGTCCTCCTGGTCCTGGCATC | CGTGCTGTCTCAAGGTACTGTCTG |
| Col-X | ATGCCGCTTGTCAGTGCTAACC | GGGTCGTAATGCTGCTGCCTATTG |
| Fabp4 | CGCAGACGACAGGAAGGTGA | TCCACCACCAGCTTGTCACC |
| GAPDH | TGTGTCCGTCGTGGATCTGA | TTGCTGTTGAAGTCGCAGGAG |
| GDF11 | GCGTCACATCCGTATCCGTTCAC | GCTCTGTGGCTGGCGAAACC |
| Mmp13 | ACTTCACGATGGCATTGCTG | CATAATTTGGCCCAGGAGGA |
| Perilipin1 | AACCCTGCTGGATGGAGACCTC | TGGGCTTCTTTGGTGCTGTTGTAG |
| PPARγ | GCCTGCGGAAGCCCTTTGGTGAC | TCAGCAAGCCTGGGCGGTCTCC |
Alp, alkaline phosphatase; Cebpα, CCAAT/enhancer-binding protein α; Col-II, type II collagen; Col-X, type X collagen; Fabp4, fatty acid binding protein 4; GAPDH, glyceraldehyde-3-phosphate dehydrogenase; GDF11, growth differentiation factor 11; MMP13, matrix metalloproteinase 13; PPARγ, peroxisome proliferator-activated receptor gamma.