. 2022 Jul 25;11(7):503–512. doi: 10.1302/2046-3758.117.BJR-2021-0358.R2

Table I.

Primers for the genes associated with osteogenic differentiation.

Target	Forward	Reverse
Runx2	5'-CGCCTCACAAACCACAG-3'	5'-AATGACTCGGTTGGTCTCGG-3'
OCN	5'-CAACCCCAATTGTGACGAGC-3'	5'-AACGGTGGTGGCATAGATGC-3´
ALP	5'-GTTTTCTGTTCTGTAAGACGGG-3'	5'-GCCGTTAATTGACGTTCCGA-3´
OPN	5'-GGCTTACGGACTGAGGTCAA-3'	5'-AGGTCCTCATCTGTGGCATC-3´
GAPDH	5'-TAGCAACTTTGGCATCGTGG-3'	5'-GGGCCATCCACAGTCTTCTG-3´

ALP, alkaline phosphatase; GAPDH, glyceraldehyde-3-phosphate dehydrogenase; OCN, osteocalcin; OPN, osteopontin; Runx2, runt-related transcription factor 2.