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. Author manuscript; available in PMC: 2022 Aug 4.
Published in final edited form as: Anal Chem. 2021 Apr 21;93(17):6629–6637. doi: 10.1021/acs.analchem.0c04220

Figure 2. 2D 1H{15N}-HSQC spectrum of 15N1-cholamine derivatives of 29 standards mixture.

Figure 2.

The standard mixture was reacted for 2 h at 60° with 15N1-cholamine and DMTMM in ratios of 1: 15: 60 in H2O as described in the Experimental Section. The spectrum was recorded at 16.45 T and processed as described in the Experimental Section. Assignment of carboxyl-containing metabolites mixture tagged with 15N1-cholamine: 1. Leu, 2. Ile, 3. Val, 4. Ala, 5. Glu, 6. Gln, 7. Asp, 8. Gly, 9. Phe, 10. His, 11. Tyr, 12. Trp, 13. Ser, 14. Thr, 15. Cys, 16. Cystine, 17. N-acetyl-Asp, 18. Lactate, 19. Pyruvate, 20. Formate, 21. Acetate, 22. Fumarate, 23. Citrate, 24. Malate, 25. Succinate, 26. α-KG, 27. 3-phosphoglycerate, 28. GSH, 29.