TABLE 1:
2D 1H{15N}-HSQC quantification of a mixture of 13C-acetate isotopomers.
| Mixtures of acetate isotopomers a | Reaction ratios b | Ratios determined by 2D 1H{15N} HSQC c |
|---|---|---|
| 13CH312COOH + 12CH313COOH | 1:1.24 | 1:1.28 |
| 12CH312COOH + 13CH312COOH + 12CH313COOH | 1:1:1 | 1:1:1.14 |
| 12CH312COOH+13CH313COOH | 1:1 | 1:1.21 |
| 12CH313COOH + 13CH313COOH | 1:1.2 | 1:1.3 |
a
Isotopomer mixtures were derivatized with 15N-cholamine and analyzed as described in the Methods.
b
Isotopomer ratios used for derivatization as determined by methyl peaks quantified in 1D NMR
c
Isotopomer ratios determined by volume integration of cross-peaks in the high-resolution 1H{15N}-HSQC spectrum; estimated errors on volume integration were < 10%