Figure 1.

(a) Primary embryonic rat hippocampal neurons in culture labelled with MAP2 (neuronal stain) and DAPI (nuclear stain). Patient serum antibodies are detected binding to the surface of neuronal cell bodies and processes (top row). In contrast, healthy control serum does not show any binding in the neuronal cultures (bottom row). Scale bar = 10 mm. (b) Representative immunofluorescence images of a cell-based assay. HEK cells transiently transfected with NR1 and labelled with DAPI (nuclear stain) shows cell surface binding with patient serum (top row). Healthy control serum does not show any binding (bottom row). Scale bar = 10 mm