FIG. 5.

Fluorescence emission spectra of BADH from P. aeruginosa. Fluorescence emission spectra in the absence (solid line) and presence (dashed line) of 0.5 mM NADP⁺ are shown. The enzyme (164 μg/ml) was in potassium phosphate buffer (pH 6.9) containing 0.1 mM EDTA, 20% (wt/vol) sucrose, and 25 mM KCl.