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Purification of BADH from P. aeruginosaa
| Purification step | Total protein (mg) | Total activity (U) | Sp act (U/mg of protein) | Purifi-cation (fold) | Yield (%) |
|---|---|---|---|---|---|
| Cell extract | 172.0 | 178 | 1.0 | 1.0 | 100 |
| Q-Sepharose Fast Flow | 23.0 | 177 | 7.7 | 7.7 | 99 |
| 2′,5′-ADP–Sepharose | 0.8 | 117 | 146.3 | 146.3 | 66 |
Results are from a typical purification starting with 1.8 liters of bacterial culture in stationary phase.
