| Source organism |
Elizabethkingia anopheles NUHP1 |
Elizabethkingia meningosepticum CCUG 26117 |
| DNA source |
Dr Yang Liang (Nanyang Technological University, Singapore) |
ATCC 13253 |
| Forward primer |
5′-CTCACCACCACCACCACCATATGGAAAAAGTACGGGTACGTTTTG-3′ |
| Reverse primer |
5′-ATCCTATCTTACTCACTTATTTTAAAGTTTCAATTGCTTTATTAATTC-3′ |
| Expression vector |
pBG1861 |
BG1861 |
| Expression host |
E. coli BL21(DE3)R3 Rosetta cells |
E. coli BL21(DE3)R3 Rosetta cells |
| Complete amino-acid sequence of the construct produced |
MAHHHHHHMEKVRVRFAPSPTGPLHLGGVRTALYDYLFAKHNGGDFILRIEDTDTQRYVPGSEEYIMEALEWIGMVPDESPKHGGPYAPYRQSERRDIYDRYTEQILKTDYAYLAFDTPEELDQIRAEFEARGDVFAYNYETRNRLRNSISLPEEEVKKLLEEKTPYVIRFKMPLDRIINLNDIIRGKFSVNTNTLDDKVLVKNDGMPTYHFANIIDDHEMKITHVIRGEEWLPSMALHVLLYEAMGWDAPEFAHLSLILKPEGKGKLSKRDGDKFGFPVFPLNFTDPATGNTSAGYREEGYLPEAFINMVAMLGWSPADNKEIVSMDEMIKEFDLNKVHKAGARFSAEKAKWFNQQYLQLMSNEAILPEFKKVLAENNVEVSDEKALKIIGLMKERATFVKDIYNDGKFFFHAPESFDEKASKKAWSPETAVLMQELTEAISSLDFKAEIIKESIHHLAEAKGLGMGKVMMPLRLSLVGELKGPDVPDLMEMIGKEETISRINKAIETLK |
MAHHHHHHMEKVRVRFAPSPTGPLHLGGVRTALYDYLFAKHNGGDFILRIEDTDTQRYVPGSEEYIMEALEWIGMIPDESPKHGGPYAPYRQSERRAIYDKYTEQILKTDYAYLAFDTPEELDQIRAEYEAKGDVFAYNYETRHRLRNSISLPEDEVKKLLDEKTPYVIRFKMPLDRIINLNDIIRGKFSVNTNTLDDKVLVKNDGMPTYHFANIIDDHEMKITHVIRGEEWLPSMALHVLLYEAMEWNAPEFAHLSLILKPEGKGKLSKRDGDKFGFPVFPLNFTDPATGNTSAGYREEGYLPEAFINMVAMLGWSPADNKEIISMDEMIKEFDLHKVHKAGARFSAEKAKWFNQQYLQMMSNEAILPEFKTILSNNSIEISDEKALRIIGLMKERATFIKDIYNDGKFFFHAPESYDEKAAKKAWSPETAALMQEVNNAITTVDFKADTIKESLHHLTEEKGLGMGKVMMPLRLSLVGELKGPDVPELMEIIGKEESVSRITKAIETLK |
