| Method |
Sitting-drop vapor diffusion |
Sitting-drop vapor diffusion |
| Plate type |
96-well, Compact 300, Rigaku |
96-well, Compact 300, Rigaku |
| Temperature (K) |
290 |
290 |
| Protein concentration (mg ml−1) |
18.25 |
16.23 |
| Buffer composition of protein solution |
25 mM HEPES pH 7.0, 500 mM NaCl, 5% glycerol, 2 mM DTT, 0.025% sodium azide |
| Composition of reservoir solution |
JBScreen JCSG++ HTS A5: 0.2 M magnesium formate, 20%(w/v) PEG 3350 |
MCSG1 E10: 200 mM ammonium tartarate dibasic, 20%(w/v) PEG 3350 |
| Volume and ratio of drop |
0.4 µl protein plus 0.4 µl reservoir (1:1) |
0.4 µl protein plus 0.4 µl reservoir (1:1) |
| Volume of reservoir (µl) |
80 |
80 |
| Cryoprotectant |
20% ethylene glycol |
None |
