Skip to main content
. Author manuscript; available in PMC: 2023 Mar 7.
Published in final edited form as: Mol Pharm. 2022 Feb 2;19(3):775–787. doi: 10.1021/acs.molpharmaceut.1c00373

Figure 2. Evaluation of non-specific interactions for grafted antibody variants in physiological conditions (pH 7.4, PBS).

Figure 2.

The antibody variants were evaluated using two ELISAs (#1 and #2), one with (A) with six different reagents (cardiolipin, LPS, KLH, ssDNA, dsDNA and insulin) and the second assay with (B) baculovirus particles (BVP). The variants were also evaluated using two flow cytometry (Polyspecificity Particle, PSP) assays, one with (C) soluble membrane proteins (SMP, PSP #1) and second one with (D) ovalbumin (OVA, PSP#2). ELISA and PSP assay values were normalized on a scale of 0 to 1 using adalimumab and bococizumab as negative and positive controls for nonspecific binding, respectively. The parental antibodies are indicated in open circles, while the negatively charged HCDR3s are indicated in filled circles and the positively charged HCDR3s are indicated in filled triangles. The values reported are averages for three independent experiments, and the error bars are standard deviations.