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. Author manuscript; available in PMC: 2023 Mar 7.
Published in final edited form as: Mol Pharm. 2022 Feb 2;19(3):775–787. doi: 10.1021/acs.molpharmaceut.1c00373

Figure 5. Antibodies with intermediate Fv isoelectric points display the best combination of formulation and physiological colloidal properties.

Figure 5.

Antibodies with low isoelectric points display low levels of non-specific interactions in physiological conditions but weakly repulsive self-interactions in formulation conditions. Conversely, antibodies with high isoelectric points display highly repulsive self-interactions in formulation conditions but high levels of non-specific interactions in physiological conditions. Intermediate isoelectric points of 7.5–9 for Fv provide an attractive balance of strongly repulsive self-interactions in formulation conditions and low-to-moderate levels of non-specific binding in physiological conditions. Y-axes represents the average values from the two (A) ELISA and (B) PSP assays used to evaluate non-specific interactions. The dotted lines are defined in Fig. 4.