Table 1.
Summary of the four clinical-stage antibodies evaluated in the study. The most homologous human germlines for the VH and VL domains are noted. The antibodies display a range of net charges and isoelectric points. Antibody charge values were calculated at pH 7.4 using values of −1 for Asp and Glu, +1 for Arg and Lys, and +0.1 for His.
| Antibody framework | pI | Net charge (pH 7.4) | Closest human germline | |||||
|---|---|---|---|---|---|---|---|---|
| VH | VL | Fv | VH | VL | Fv | VH | VL | |
| hOKT3 | 9.2 | 9.2 | 9.3 | 5.2 | 4 | 9.2 | IGHV1–46 | IGKV3–20 |
| Trastuzumab | 8.0 | 8.6 | 8.6 | 1.1 | 2.1 | 3.1 | IGHV3–66 | IGKV1–39 |
| Siltuximab | 6.5 | 7.9 | 7.8 | −1 | 1 | 0 | IGHV3–23 | IGKV3–11 |
| Lebrikizumab | 7.9 | 4.9 | 5.4 | 1 | −2.9 | −1.9 | IGHV2–70 | IGKV4–1 |