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. 2022 Jul 22;48(3):164. doi: 10.3892/or.2022.8379

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Copyright: © Zhang et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 3. — miR-491-3p directly inhibits the expression of FGF5. (A) TargetScan 7.1 online prediction software revealed that FGF5 possessed a binding site for miR-491-3p in the 3′-UTR region. (B) Dual luciferase reporter gene assay verified that FGF5 is a target gene of miR-491-3p. (C) FGF5 mRNA expression in clinical samples of patients with NSCLC was detected by RT-qPCR. Pearson's correlation coefficient analysis was employed to identify the correlation between miR-491-3p and FGF5 mRNA in tumor tissues of patients NSCLC. (D) RT-qPCR and western blotting indicated that miR-491-3p suppressed the expression of FGF5 mRNA and protein. **P<0.01. miR, microRNA; FGF5, fibroblast growth factor 5; UTR, untranslated; RT-qPCR, reverse transcription-quantitative PCR; WT, wild-type; MUT, mutant; NC, negative control.