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. 2022 Jul 25;48(3):166. doi: 10.3892/or.2022.8381

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Copyright: © Li et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 2. — Expression of Cx32 mRNA is decreased in HCC cell populations with higher stem cell characteristics. (A and B) Cx32 mRNA in CD133+, EpCAM+ liver CSCs and CD133-, EpCAM-non-liver CSCs sorted from primary HCC cells was analyzed by qPCR. *P<0.05 vs. the CD133- or EpCAM- group. (C) Cx32 mRNA in HCC spheres and attached cells obtained from primary HCC cells was detected by qPCR, *P<0.05 vs. the attached cells. (D and E) Expression level of Cx32 in CD133+, EpCAM+ liver CSCs and CD133-, EpCA- non-liver CSCs sorted from spheres of HepG2 and HCCLM3 cells was analyzed by qPCR. *P<0.05 vs. the CD133- or EpCAM- group. (F) Cx32 mRNA levels were detected in spheres of HepG2 and HCCLM3 cells and corresponding adherent cells. *P<0.05 vs. the attached cells. Cx32, connexin 32; HCC, hepatocellular carcinoma; EpCAM, epithelial cell adhesion molecule; CSCs, cancer stem cells; qPCR, quantitative PCR.