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Primers used for quantitative PCR.
| Primer sequences(5′-3′) | ||
|---|---|---|
|
|
||
| Gene name | Forward primer | Reverse primer |
| β-actin | CATCCACGAAACTACCTTCAACTCC | GAGCCGCCGATCCACACG |
| Sox-9 | AGGAAGTCGGTGAAGAACGG | AAGTCGATAGGGGGCTGTCT |
| CD133 | TGGATGCAGAACTTGACAACGT | ATACCTGCTACGACAGTCGTGGT |
| EpCAM | CGCAGCTCAGGAAGAATGTG | TGAAGTACACTGGCATTGACGA |
| Nanog | AATACCTCAGCCTCCAGCAGATG | TGCGTCACACCATTGCTATTCTTC |
| Oct4 | GTGTTCAGCCAAAAGACCATCT | GGCCTGCATGAGGGTTTCT |
| c-Myc | CCCTCCACTCGGAAGGACTA | GCTGGTGCATTTTCGGTTGT |
