Fig. 2.

Gene and protein expression of the monoamine transporters in placental cells. Results of quantitative PCR (A) and Western blot (B) analysis of SERT, NET, DAT, and OCT3 showing fold-differences in expression levels of the genes between undifferentiated (CTB) and differentiated (STB) PHT and BeWo cells. Gene expression results were normalized to the geometric mean of YWHAZ and B2M expression, whereas protein expression was normalized to the total protein, visualized by Ponceau S staining. Presented data are means ± SD (n = 4), with indications of statistical significance obtained from paired t-tests: *p ≤ 0.05, **p ≤ 0.01, ***p ≤ 0.001, ND = not detected