Fig. 7.

Knockdown of Dnmt3a promotes osteogenic differentiation of DOP-ASCs. A: Alizarin Red-S staining showing that the formation of mineralized nodules was the highest in the Dnmt3a shRNA group and the lowest in the Dnmt3a LVRNA group (osteoinduction for 21 days). B–D: mRNA and protein levels of osteogenesis-related molecules were upregulated in the Dnmt3a shRNA group compared with the other three groups (osteoinduction for 4 days). E–G: mRNA and protein levels of Wnt signaling pathway markers were recovered by knockdown of Dnmt3a. Data represent the mean ± SD of at least three independent experiments, *P < 0.05, **P < 0.01