Table 2.
Cell division defects found in CRS-depleted HeLa cells
| Mock | Scramble | BAF53a | CFDP1 | GAS41 | MRG15 | p400 | Tip60 | YL1 | ||
|---|---|---|---|---|---|---|---|---|---|---|
| Metaphase | MS | 1.43 ± 1.47 | 0 ± 0 | 3.12 ± 3.35 | 0.43 ± 0.74 | 12.77 ± 3.13*** | 3.23 ± 2.01 | 11.95 ± 0.74*** | 10.77 ± 3.27*** | 10.49 ± 2.80*** |
| CM | 2.03 ± 1.76 | 0.79 ± 1.37 | 29.00 ± 2.06*** | 23.90 ± 6.11*** | 31.60 ± 3.00*** | 7.16 ± 1.85*(Scramble) | 2.74 ± 0.85 | 29.80 ± 3.10*** | 34.98 ± 4.72*** | |
| Telophase | CB | 0.87 ± 1.52 | 0.56 ± 0.98 | 15.86 ± 0.57*** | 30.16 ± 1.96*** | 1.80 ± 0.97 | 4.13 ± 0.95*** | 1.29 ± 0.14 | 4.55 ± 3.75*** | 1.83 ± 0.39 |
| LIB | 0.44 ± 0.20 | 0.62 ± 0.30 | 30.27 ± 2.79*** | 0.57 ± 0.51 | 9.41 ± 1.54*** | 3.19 ± 1.74*** | 0.84 ± 0.36 | 4.88 ± 2.75*** | 8.32 ± 3.29*** | |
| MC | 1.40 ± 1.03 | 1.55 ± 1.21 | 5.96 ± 0.90*** | 4.02 ± 1.99*** | 2.89 ± 0.90*** | 6.87 ± 1.36*** | 4.98 ± 0.89*** | 3.09 ± 1.27*** | 2.47 ± 0.65*** |
MS multipolar spindles, CM chromosome misalignments, CB chromatin bridges, LIB long intercellular bridges, MC multinucleated cells
The results are expressed as mean ± SD values from three independent replicate experiments. Experimental groups were compared with control groups (mocks and scrambles) by Fisher’s exact test. *=P < 0.05; **P= < 0.005; and ***=P < 0.0005
*(Scramble)=This means that it is statistically significant only with the scramble