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. 2022 Aug 3;13:394. doi: 10.1186/s13287-022-03100-x

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© The Author(s) 2022

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 8 — MiR-146a directly regulates the expression of Nr4a3. A, B The expression of miR-146a-5p (A) and Nr4a3 (B) after MSC-EVs treatment for 24 h in 661 W cells. C, D The expression of miR-146a-5p (C) and Nr4a3 (D) after overexpressing miR-146a-5p by using the mimic or blocking it using inhibitor in 661 W cells. E, F The expression of miR-146a-5p(E) and Nr4a3 (F) after MSC-EVs treatment in BV2 cells. G, H The expression of miR-146a-5p (G) and Nr4a3 (H) after using the mimic or inhibitor in BV2 cells. The expression of Nr4a3 followed the changes of miR-146a-5p. I The luciferase activity of miR-146a-5p and Nr4a3 or with two forms mutated at the predicted binding sites of Nr4a3. Values were normalized to that of Nr4a3-negative control. J Overexpressing miR-146a in BV2 cells downregulated expression of the LPS-induced pro-inflammatory factors IL-1β, TNF-α and IL-6. Data are shown as mean ± SEM. One-way ANOVA and Student’s t test were applied for statistics. All experiments were repeated for 3 times