Table 1.
All datasets used in this study
| Dataset (name) | Ticks (n) | Life stages | Locations in the Netherlandsa | Year of sampling | Molecular technique | Source |
|---|---|---|---|---|---|---|
| Microbiome | 655 in 133 pools | Larvae, nymphs, adult females and males | AW, DK, ST, BU, HD, ZM (n = 6) | 2016 | 16S rRNA sequencing | This study |
| Symbiont | 16,555 | Nymphs | AW, DK, ST, BU, HD, ZM, PD, SD, VH, VA, MH, HM, BB, PW, DW, EN, RB, VL, KB (n = 19) | 2013–2014 | qPCR targeting: R. helvetica, S. ixodetis, M. mitochondrii, Rickettsiella spp. | This study |
| Pathogen | 13,967 | Nymphs | AW, DK, ST, BU, HD, ZM, PD, SD, VH, VA, MH, HM, BB, PW, DW, EN, RB, VL, KB (n = 19) | 2013–2014 | qPCR targeting: B. burgdorferi s.l., A. phagocytophilum, N. mikurensis | [22] |
| Transmission mode | 1130 | Larvae, nymphs, adult females and males | AW, ST (n = 2) | 2019 | qPCR targeting: R. helvetica, S. ixodetis, M. mitochondrii, Rickettsiella spp., B. burgdorferi s.l., A. phagocytophilum, N. mikurensis | This study |
aThe abbreviations of locations are explained in Additional file 1: Table S1