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. Author manuscript; available in PMC: 2023 Aug 1.
Published in final edited form as: J Leukoc Biol. 2022 Feb 9;112(2):221–232. doi: 10.1002/JLB.4HI0921-492R

Figure 3. Effects of T cell Receptor Activation on the Splenic Innate Immune Response to LPS.

Figure 3.

Abbreviations for the five treatment groups of C57Bl/6 mice as detailed in Fig. 1. Data obtained 24 hrs. post treatment. Gating: Neutrophils: FSC/SSC, singlets, Live, CD11b+/Ly6G+; also Ly6Cint; Classical Dendritic Cells: FSC/SSC, singlets, Live, CD11c+/CD11b+/−, CD11c+/MHCII+, MHCI, MHCII, CD86, CD80, CD40, IL12p40. Monocyte-derived Dendritic Cells (MoDCs): FSC/SSC, singlets, Live, CD11c−/CD11b+, CD11b+/Ly6G−, CD11b+/MHCII+, MHCI, MHCII, CD86, CD80, CD40. Full gating strategy shown in Supp. Fig. 5B. Data as mean ± standard deviation. Significance determined using one-way ANOVA with Dunnett correction for multiple comparisons. * = p<0.05 relative to Iso, # = p<0.05 LPSHi vs. CD3LPSLo. Figures representative of at least two independent experiments.

A. Number of neutrophils obtained from the spleen. N=6/group.

B. Number of classical dendritic cells obtained from the spleen. N=6/group.

C. MHCI MFI for cDCs and MoDCs. N=3/group.

D. MHCII MFI for cDCs and MoDCs. N=3/group.

E. CD86 MFI for cDCs and MoDCs. N=3/group.

F. CD80 MFI for cDCs and MoDCs. N=3/group.

G. CD40 MFI for cDCs and MoDCs. N=3/group.

H. Number of splenic classical dendritic cells producing IL12p40 ex vivo without stimulation. N=6/group.