Figure 7. Effects of Cytokine Blockade on the T cell and Innate Immune Response to TCR modulated LPS Challenge.

Iso, CD3LPSLo, or LPSHi mice treated with blocking antibodies to IFNγ, TNFα or IL12p40 as per previous figure on day 1 of challenge and every 3 days following. Mice studied 24 hrs. following treatment. Gating: T cells: FSC/SSC, singlets, Live, CD90/CD4, CD90/CD8, CD69, Ki67, Granzyme B, PD1. Classical Dendritic Cells: FSC/SSC, singlets, Live, CD11c⁺/CD11b^+/−, CD11c⁺/MHCII⁺, MHCII, CD40. Data as mean ± standard deviation, full gating strategy shown in Supp. Fig. 5B and 6. Significance determined using one-way ANOVA with Dunnett correction for multiple comparisons; * = p<0.05 relative to CD3LPSLo. Figures representative of at least two independent experiments.

A. Number of CD69⁺ CD8 T cells obtained from the spleen. N=3–4/group.

B. Number of Ki67⁺ CD8 T cells obtained from the spleen. N=3–4/group.

C. Total splenic CD8 T cells producing IFNγ ex vivo without stimulation. Cells treated with Brefeldin A for four hours prior to intracellular cytokine staining. N=3–4/group.

D. Number of PD1⁻CD69⁺ CD8 T cells obtained from the spleen. N=3–4/group.

E. Number of Granzyme B⁺ CD8 T cells obtained from the spleen. N=3–4/group.

F. Number of Granzyme B⁺ CD4 T cells obtained from the spleen. N=3–4/group.

G. CD40 MFI for cDCs. N=6/group.

H. MHCII MFI for cDCs and MoDCs. N=6/group.