Fig. 3.

Combination of EZH2 and HDAC inhibitors induce NK and T cell-mediated cytotoxicity in HER2⁺ cells. (A) Schematic overview of coculture experiment of PBMC expanded NK and T cells with epigenetic inhibitor-treated BC cell lines. (B) Representative data showing D9/ENT or EPZ/ENT boosts NK-mediated cytotoxicity and (C) dendritic cell-primed specific CD8⁺ T-mediated cytotoxicity of SKBR3 and MDA-MB-231 cells. Data are expressed as means ± SEM of three technical replicates, representative of three independent experiments. P values were calculated with two-way ANOVA with Sidak’s multiple comparison test, ****P < 0.0001, ns, not significant. (D) Schematic overview of coculture experiment of mouse immune cells and epigenetic inhibitors treated mouse cancer cells. (E) Representative lysis data showing D9/ENT or EPZ/ENT boosts the 4T1-HER2 cell lysis by splenocytes, and (F) NK cells and (G) T cells isolated from 4T1-HER2 tumor-bearing Balb/c mouse. Data are expressed as means ± SEM of three technical replicates, representing two independent experiments. P values were calculated with one-way ANOVA with Tukey’s multiple comparison test, *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001, ns, not significant.