Fig. 5.

TFEB does not regulate autophagy and lysosome activity in Treg cells. (A) WT or Tcfeb-deficient naïve CD4⁺ T cells were differentiated under Treg condition for 3 d in the absence or presence of rapamycin (100 nM) or BafA1 (100 nM). LC3 puncta was stained with immunofluorescent antibodies and analyzed with confocal microscopy, and the amounts were quantified with Image J. (B) WT or Tcfeb-deficient naïve CD4⁺ T cells were differentiated under Treg conditions, in the presence of rapamycin (100 nM), or 3-MA (5 mM) for 3 d. Representative flow cytometry plots and frequencies of CD4⁺Foxp3⁺ Treg cells were determined. ns, no significance; *P < 0.05, **P < 0.01, ***P < 0.001. Data are representative of three experiments. Data are means ± SEM and were analyzed by two-way ANOVA.