Table 3.
Thermodynamic properties of nanobodies described in this work binding to the RBD
| Nanobody | Thermodynamics (kJ/mol) | Kinetics | |||
|---|---|---|---|---|---|
| KD (nM) | ΔH | −TΔS | ΔG | t1/2 (s) | |
| H11* | 930 (±47) | −47 | 12 | −34 | NM |
| H11-A10 | 60 (±4.6) | −83 | 42 | −41 | 14 |
| H11-H6 | 57 (±4.5) | −80 | 39 | −41 | 25 |
| H11-B5 | 53 (±5.3) | −68 | 27 | −41 | 14 |
| H11-D4* | 39 (±2) | −76 | 34 | −42 | 26 |
| H11-H4* | 12 (±1.5) | −69 | 23 | −46 | 29 |
| H4_R52E | ND† | NP | NP | NP | NP |
| H4_R52E with E484R in RBD | ND† | NP | NP | NP | NP |
| H4_R52A | ND† | NP | NP | NP | NP |
| H4_R52K | ND† | NP | NP | NP | NP |
| H4_Y101A | 1,079 ± 193 | −42 | 8 | −34 | NP |
| H4_Y104S | 409 ± 91 | −46 | 9 | −37 | NP |
| H4_Y104F | 40 ± 12 | −57 | 15 | −42 | 9 |
| H11_V101Y_R103S | 5,221 | −46 | 16 | −30 | NP |
| H11-H4 T99Y (B5 single hybrid)‡ | NM | NP | NP | NP | NP |
| H11-H4 Q98R H100E (D4 hybrid) | 18 (±1) | −69 | 25 | −44 | 29 |
| H11-H4 Q98R, T99Y, H100E (B5-D4 hybrid)‡ | NM | NP | NP | NP | NP |
NM, not measured reliably; ND, no binding detected; NP, experiment not performed.
*These data are from previous publications (29).
†SPR did not detect any binding; no further analysis carried out.
‡SPR showed weak binding with KD > 1000 nM; no further analysis was carried out.